Evaluation of Cross-Species Transferability of SSR Markers in Foeniculum vulgare
نویسندگان
چکیده
منابع مشابه
Novel and highly informative Capsicum SSR markers and their cross-species transferability.
This study was undertaken primarily to develop new simple sequence repeat (SSR) markers for Capsicum. As part of this project aimed at broadening the use of molecular tools in Capsicum breeding, two genomic libraries enriched for AG/TC repeat sequences were constructed for Capsicum annuum. A total of 475 DNA clones were sequenced from both libraries and 144 SSR markers were tested on cultivated...
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The cross-species transferability of 20 microsatellite markers was tested in the genus Lippia. Eleven markers were polymorphic after screening 19 accessions of Lippia sidoides and Lippia gracilis maintained in the Active Germplasm Bank (AGB) from Universidade Federal de Sergipe. Additionally, 40 accessions of Lippia spp were collected in Sergipe to increase the germplasm bank. A total of 23, 22...
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Cross-species amplification of Simple Sequence Repeats (SSRs) loci is considered as a cost-effective approach for developing microsatellite markers for new species. We examined the transferability of eucalyptus SSR loci to the economically important members of the Casuarinaceae family. Ten primer pairs targeting (GA)n, (CTT)n, (TGA)n and (GAA)n motifs were screened in Allocasuarina littoralis, ...
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Expressed sequence tags (ESTs) are important resource for gene discovery, gene expression and its regulation, molecular marker development, and comparative genomics. We procured 10000 ESTs and analyzed 267 EST-SSRs markers through computational approach. The average density was one SSR/10.45 kb or 6.4% frequency, wherein trinucleotide repeats (66.74%) were the most abundant followed by di- (26....
متن کاملDevelopment and cross-species transferability of EST-SSR markers in Siberian wildrye (Elymus sibiricus L.) using Illumina sequencing
Siberian wildrye (Elymus sibiricus L.) is a perennial, self-fertilizing grass that plays an important role in animal husbandry and environmental sustenance. However, the transcriptomic and genomic information on this species is very limited, which hinders genetic and breeding studies. In the present study, 76,686,804 clean reads were generated from 11 different tissue samples of E. sibiricus by...
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ژورنال
عنوان ژورنال: Plants
سال: 2020
ISSN: 2223-7747
DOI: 10.3390/plants9020175